Journal: Nature Communications

Article Title: Somatic gene delivery faithfully recapitulates a molecular spectrum of high-risk sarcomas

doi: 10.1038/s41467-025-60519-5

Figure Lengend Snippet: a Analysis scheme of cross-species sarcoma analysis based on RNA sequencing. b tSNE visualization based on cross-species transcriptome analysis of mouse ( n = 63) and human ( n = 299) sarcoma specimens. SS synovial sarcoma, aRMS alveolar sarcoma, IFS infantile fbrosarcoma, ASPS alveolar soft part sarcoma, RMS rhabdomyosarcoma, MPNST malignant peripheral nerve sheath tumor, MRT malignant rhabdoid tumor, UPS Undifferentiated pleomorphic sarcoma, MFS myofibroblastic tumor, LMS leiomyosarcoma, LPS liposarcoma, GIST gastrointestinal stromal tumor. Source data are provided as a Source data file.

Article Snippet: PCR products were purified using NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL, 740.609.250) and subsequently subjected to Sanger sequencing through Microsynth Seqlab services, utilizing the corresponding forward primer.

Techniques: RNA Sequencing

Journal: Nature Communications

Article Title: An expression atlas of variant ionotropic glutamate receptors identifies a molecular basis of carbonation sensing

doi: 10.1038/s41467-018-06453-1

Figure Lengend Snippet: IR56d, IR25a and IR76b are required for sensory responses to carbonation but not sucrose. a Schematic of the Ir56d locus (single exon in grey), showing the position of the CRISPR target and the sequence of the Ir56d mutant alleles in these regions. PAM protospacer adjacent motif. b Raw fluorescence of GCaMP3 expressed in IR56d neurons, and colour-coded images (reflecting the maximal GCaMP3 fluorescence intensity changes; scale bars for each genotype on the right) in control, Ir56d 1 , Ir25a 2 and Ir76b 2 mutant flies stimulated with 1 M sucrose and a carbonated solution (as in Fig. ). For genotypes, see c . c Quantification of changes in ∆ F / F in the ROIs as shown in Fig. upon application of the indicated chemicals to the proboscis of the indicated genotypes: IR56d: Control: w;Bl/+; UAS-GCaMP3,Ir56d-Gal4/+ ( n = 8); Mutant: w;Ir56d 1 /Ir56d 1 ; UAS-GCaMP3,Ir56d-Gal4/+ ( n = 11); Rescue: w;Ir56d 1 ,UAS-Ir56d/Ir56d 1 ;UAS-GCaMP3,Ir56d-Gal4/+ ( n = 10). IR25a: Control: w;Bl/+; UAS-GCaMP3,Ir56d-Gal4/+ ( n = 11); Mutant: w;Ir25a 2 /Ir25a 2 ;UAS-GCaMP3,Ir56d-Gal4/+ ( n = 11); Rescue: w;Ir25a 2 ,UAS-Ir25a/Ir25a 2 ; UAS-GCaMP3,Ir56d-Gal4/+ ( n = 9). IR76b: Control: w;Ir76b-Gal4/CyO;UAS-GCaMP3/TM6B ( n = 8); Mutant: w;Ir76b-Gal4/+; UAS-GCaMP3,Ir76b 2 /Ir76b 2 ( n = 10); Rescue: w;Ir76b-Gal4,UAS-Ir76b/+;UAS-GCaMP3,Ir76b 2 /Ir76b 2 ( n = 7). We used Ir76b-Gal4 in the rescue experiments of the Ir76b mutant due to constraints of the chromosomal location of the relevant transgenes; although Ir76b-Gal4 is more broadly-expressed than Ir56d-Gal4 , the AMS1 and PMS4 projections are still easily recognisable. For the statistical analysis the response data for each stimulus were compared with water; only significant differences are shown: * P < 0.05, ** P < 0.01, *** P < 0.001 (Wilcoxon rank sum test with Bonferroni correction for multiple comparisons)

Article Snippet: The template was transcribed in vitro with T7 polymerase, RNA was microinjected into vas-Cas9 flies (expressing Cas9 specifically in the germline ) and mutations in the target sequence region screened by Genetic Services, Inc. After establishment of homozygous mutant lines, mutations were reconfirmed by Sanger sequencing.

Techniques: CRISPR, Sequencing, Mutagenesis, Fluorescence, Control